Family members of those being commemorated were invited to take part in the ceremony, including Patrick Walsh, a godson of Detective Garda Colm Horkan.
Mr Horkan was the last member of the force to die on duty. The 49-year-old was shot dead in Castlerea, County Roscommon, in June 2020.
His godson delivered a reading in his honour.
‘Brave and selfless’
In her address, the minister for justice said the death of every Garda member in the line of duty was “both a huge loss to their family and friends, but also to the wider communities in which they lived and worked.
Ms McEntee also acknowledged the dangers of the job and the stress it can put on officers’ families.
“You live in the knowledge that your loved one may be put at personal risk when they go to work, and I want to thank you for supporting your family members, our Garda members, in the brave and selfless work that they do,” she said.